Bug #4047
closedctfind4 stigmatism definition has changed
0%
Description
Found this from a highly stigamtic image, attached.
Running through Appion ctffind3 gives
logfiles final ctf DFMID1 9171.05, DFMID2 5113.67, ANGAST 55.54
ctfvalues.log nominal=1.0e-6, bestdef=5.1e-7, def_1=9.2e-07, def_2=5.1e-7, astig_angle=34.4, cross_corr=0.153
imageviewer shows def_1=5.1e-7, def_2=9.2e-7, astig_angle=-55.57
2D and 1D plot from Appion shows good match.
-----------------Running through Appion ctffind4 gives
ctffind4 output:
- Estimated defocus values:9159.57, 5109.79 Angstroms
- Estimated azimuth of astigmatism: 34.18 degrees
database record and imageviewer shows
def_1=0.51 um, def_2=0.92 um, astig_amgle=34.18
2D and 1D plots appears mirrored between fit and data.
This bug is in 3.2, need fixing immediately.
Files
Updated by Neil Voss over 8 years ago
Can I get the voltage, pixel size, and Cs for the attached image? Or could you attach the result images.
I know for CTFFIND3 I need to rotate the value to get the proper astigmatism angle.
Updated by Anchi Cheng over 8 years ago
- File ctffind4result.png ctffind4result.png added
300 kV
2.7 mm Cs
2.8 Angstrom per pixel
Updated by Anchi Cheng over 8 years ago
- Description updated (diff)
Corrected in the description ctffind3 ANGAST from 45.54 to 55.54
Updated by Neil Voss over 8 years ago
- Status changed from New to Assigned
actively working on this. My CTF program fail with the error:
"!!! WARNING: Too few points to work with, probably bad defocus estimate"
Updated by Neil Voss over 8 years ago
- Status changed from Assigned to In Test
- Assignee changed from Neil Voss to Anchi Cheng
Hi Anchi, Should be working now, I tested your astigmatic image with both ctffind3 and ctffind4, both give high resolution fits now.
I added some additional changes to ctfdisplay to help on images like this.
Updated by Anchi Cheng over 8 years ago
- Status changed from In Test to Closed
Thanks Neil, it works great. The fit is better than ever. Will need
merge the sign change part to myami-beta as r19622 and to myami-3.2 as r19623
Updated by Neil Voss over 8 years ago
I made some more fixes today, but I need to test them more before I am ready to commit.