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Bug #4047
closedctfind4 stigmatism definition has changed
Start date:
03/17/2016
Due date:
% Done:
0%
Estimated time:
Affected Version:
Appion/Leginon 3.2
Show in known bugs:
No
Workaround:
Description
Found this from a highly stigamtic image, attached.
Running through Appion ctffind3 gives
logfiles final ctf DFMID1 9171.05, DFMID2 5113.67, ANGAST 55.54
ctfvalues.log nominal=1.0e-6, bestdef=5.1e-7, def_1=9.2e-07, def_2=5.1e-7, astig_angle=34.4, cross_corr=0.153
imageviewer shows def_1=5.1e-7, def_2=9.2e-7, astig_angle=-55.57
2D and 1D plot from Appion shows good match.
-----------------Running through Appion ctffind4 gives
ctffind4 output:
- Estimated defocus values:9159.57, 5109.79 Angstroms
- Estimated azimuth of astigmatism: 34.18 degrees
database record and imageviewer shows
def_1=0.51 um, def_2=0.92 um, astig_amgle=34.18
2D and 1D plots appears mirrored between fit and data.
This bug is in 3.2, need fixing immediately.
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