Project

General

Profile

Actions

Bug #4047

closed

ctfind4 stigmatism definition has changed

Added by Anchi Cheng over 8 years ago. Updated over 8 years ago.

Status:
Closed
Priority:
Immediate
Assignee:
Category:
-
Target version:
Start date:
03/17/2016
Due date:
% Done:

0%

Estimated time:
Affected Version:
Appion/Leginon 3.2
Show in known bugs:
No
Workaround:

Description

Found this from a highly stigamtic image, attached.

Running through Appion ctffind3 gives

logfiles final ctf DFMID1 9171.05, DFMID2 5113.67, ANGAST 55.54
ctfvalues.log nominal=1.0e-6, bestdef=5.1e-7, def_1=9.2e-07, def_2=5.1e-7, astig_angle=34.4, cross_corr=0.153
imageviewer shows def_1=5.1e-7, def_2=9.2e-7, astig_angle=-55.57

2D and 1D plot from Appion shows good match.

-----------------
Running through Appion ctffind4 gives
ctffind4 output:
  • Estimated defocus values:9159.57, 5109.79 Angstroms
  • Estimated azimuth of astigmatism: 34.18 degrees

database record and imageviewer shows
def_1=0.51 um, def_2=0.92 um, astig_amgle=34.18

2D and 1D plots appears mirrored between fit and data.

This bug is in 3.2, need fixing immediately.


Files

16mar16b_00005fcn.mrc (4 MB) 16mar16b_00005fcn.mrc Anchi Cheng, 03/17/2016 02:38 PM
ctffind4result.png (1.45 MB) ctffind4result.png Anchi Cheng, 03/17/2016 06:55 PM
Actions

Also available in: Atom PDF