Appion Manual¶

1. An Introduction to Appion
   1. What is Appion?
   2. System Requirements
   3. Authors
2. Complete Installation
   1. Instructions for installing CentOS on your computer
   2. Download additional Software
   3. Database Server Installation
   4. Processing Server Installation
      1. Install supporting packages
      2. Download Appion Files
      3. Perform system check
      4. Install Appion Packages
      5. Configure leginon.cfg
      6. Explanation of Sample Names
      7. Configure sinedon.cfg
      8. Install External Packages
      9. Compile FindEM
      10. Compile Ace2
      11. Compile Radermacher
      12. Install Xmipp
      13. Test Appion
   5. Web Server Installation
      1. Differences between Linux flavors
      2. Install Web Server Prerequisites
      3. Configure php.ini
      4. Install Apache Web Server
      5. Check php information
      6. Install the MRC PHP Extension
      7. Install the Web Interface
      8. Potential Problems
   6. Create a Test Project
   7. Setup Remote Processing
3. Upgrade Instructions
4. Appion User Guide
   1. Appion and Leginon Database Tools
      1. Administration
      2. User Management
      3. Project DB
      4. Image Viewers
      5. LOI - Leginon Observer Interface
      6. RCT
      7. Tomography
      8. Hole Template Viewer
   2. Appion Processing
      1. Terminology
      2. Common Workflow
      3. Processing Cluster Login
      4. Particle Selection
      5. CTF Estimation
      6. Import Tools
      7. Image Assessment
      8. Region Mask Creation