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Ab Initio Reconstruction » History » Version 8

Anke Mulder, 06/03/2010 06:56 PM

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h1. Ab Initio Reconstruction
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This section contains procedures for calculating initial models from tilted and untilted datasets.
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!http://emg.nysbc.org/attachments/277/Picture_81.png!
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h2. Tilted Data Procedures
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# [[RCT Volume|Random Conical Tilt Reconstruction (RCT Volume)]]: Obtain 3D structure from tilted data  (typically -55,0).
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# [[OTR Volume|Orthogonal Tilt Reconstruction (OTR Volume)]]: Obtain a 3D structure from data collected at 2 tilts (typically -45,0,45)
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h2. Un-Tilted Data Procedures
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# [[EMAN Common Lines|EMAN Common Lines Reconstruction]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules.
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# [[IMAGIC Angular Reconstitution|IMAGIC Angular Reconstitution]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules.
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h2. Appion SideBar Snapshot:
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!http://emg.nysbc.org/attachments/198/Picture_87.png!
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h2. Appion Composite Output Snapshot:
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!http://emg.nysbc.org/attachments/199/Picture_88.png!
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[[Particle Alignment|< Particle Alignment]] | [[Refine Reconstruction|Refine Reconstruction >]]
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