Appion

h1. Ab Initio Reconstruction

This section contains procedures for calculating initial models from tilted and untilted datasets.

h2. Tilted Data Procedures

# [[RCT Volume|Random Conical Tilt Reconstruction (RCT Volume)]]: Obtain 3D structure from tilted data  (typically -55,0).

# [[OTR Volume|Orthogonal Tilt Reconstruction (OTR Volume)]]: Obtain a 3D structure from data collected at 2 tilts (typically -45,0,45)

h2. Un-Tilted Data Procedures

# [[EMAN Common Lines|EMAN Common Lines Reconstruction]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules.

# [[IMAGIC Angular Reconstitution|IMAGIC Angular Reconstitution]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules.

h2. Appion SideBar Snapshot:

!http://emg.nysbc.org/attachments/198/Picture_87.png!

h2. Appion Composite Output Snapshot:

!http://emg.nysbc.org/attachments/199/Picture_88.png!

h2. Notes, Comments, and Suggestions:

!http://emg.nysbc.org/attachments/277/Picture_81.png!

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[[Particle Alignment|< Particle Alignment]] | [[Refine Reconstruction|Refine Reconstruction >]]

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